Academic journal article Iranian Journal of Public Health

Occurrence of Enterotoxin Gene Profile of Staphylococcus Aureus Isolated from Raw Milk of Cattle, Sheep and Goat and Pasteurized Milk Marketed in Shahrekord

Academic journal article Iranian Journal of Public Health

Occurrence of Enterotoxin Gene Profile of Staphylococcus Aureus Isolated from Raw Milk of Cattle, Sheep and Goat and Pasteurized Milk Marketed in Shahrekord

Article excerpt

Background: Nowadays, milk has been considered as one of the most important supplies to provide nutritional require- ments of human. However, milk and dairy products could be a source of contamination by different foodborne pathogens such as Staphylococcus aureus. This study was aimed to deter- mine the occurrence of genes encoding the staphylococcal enterotoxins (SEs), sea, seb, sec, sed, see, seg, seh, sei and sej in S. aureus strains isolated from raw or pasteurized milk of cattle, sheep and goats marketed in Shahrekord.

Methods: A total of 256 samples of raw milk of cattle, sheep and goat and 10 samples of pasteurized milk were collected from Shahrekord market, in Chaharmahal and Bakhtiari province, during 8 months. Multiplex PCR was performed to detect different kinds of the SEs genes of isolated strains.

Results: S. aureus was isolated from 123 (50 %) of 246 raw milk samples. The number of isolated strains of raw milk of cattle, sheep and goat were 89, (54.93 %), 18, (56.25 %) and 16, (30.76 %), respectively. The microorganism was not iso- lated from pasteurized milk samples. …

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