Academic journal article Iranian Journal of Public Health

Detection of Escherichia Coli O157: H7 in Milk by Direct Pcr

Academic journal article Iranian Journal of Public Health

Detection of Escherichia Coli O157: H7 in Milk by Direct Pcr

Article excerpt

Background: Escherichia coli O157 is the most common member of a group of pathogenic E. coli strains known vari- ously as enterohaemorrhagic or Shiga-toxin-producing organ- isms. Domestic and wild ruminants are regarded as the main natural reservoirs. In developed countries, serotype O157: H7 represents the major cause of human gastrointestinal infections. The present study was conducted to investigate the presence of E. coli O157: H7 strains and the presence of virulence genes (stx1, stx2), in isolates derived from collected milk of dairy industry.

Methods: A total number of 150 milk samples were collect- ed from dairy industry in Khuzestan, over a period of 6 months and were evaluated by cultivation in pre-enrich media tryptic soy broth contain of novobiocin and direct PCR on it. …

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