Academic journal article Iranian Journal of Public Health

Molecular Fingerprinting as a Tool for Surveillance Study of Foodborne Infections in Hospital Settings

Academic journal article Iranian Journal of Public Health

Molecular Fingerprinting as a Tool for Surveillance Study of Foodborne Infections in Hospital Settings

Article excerpt

Background: Hospital acquired infections are still a major healthcare problem in the world. Hospitals have been identi- fied as high food safety risk institutions because they serve potentially hazardous foods to vulnerable people. The aim of this study was to investigate role of hospital foods in transi- tion ofand S. aureus strains into the hospital environment.

Methods: A total of 322 samples from hospital's personnel, patients' clinical and environmental samples, and 210 samples from the hospital kitchen, including raw and cooked foods, food handlers and food processing devices were collected in a hospital in Tehran- Iran. Standard swab and food sampling methods were used in each case. Colony count of each cul- ture and biochemical or molecular identification of each iso- late was determined according to standard methods. Antimi- crobial susceptibility of each bacterial isolate was determined according to the latest clinical laboratory standard guideline. RAPD-PCR molecular typing method was used as a biotyp- ing method to analyze genetics convergence among the clini- cal and food isolates. Gelcompare II software was used for analysis of the RAPD patterns. Possible correlation between the strains from different samples was detected by comparing both RAPD patterns and resistance profiles. …

Search by... Author
Show... All Results Primary Sources Peer-reviewed

Oops!

An unknown error has occurred. Please click the button below to reload the page. If the problem persists, please try again in a little while.