Academic journal article Iranian Journal of Public Health

Prevalence of Coagulase Positive Pathogenic Staphylococcus Aureus in Mayonnaise Sauce

Academic journal article Iranian Journal of Public Health

Prevalence of Coagulase Positive Pathogenic Staphylococcus Aureus in Mayonnaise Sauce

Article excerpt

Background: This study was done to determine the preva- lence of coagulase positive pathogenic Staphylococcus aureus strains isolated from mayonnaise sauce and drug sensitivity pattern.

Methods: In this experimental study, we used of 120 packets (sachet) of mayonnaise sauce and enriched samples were streaked on Baird Parker Agar (BPA) and the plate was incu- bated at 37 °C for 24-48 hrs. Appearance of jet black colo- nies surrounded by a white halo were considered to be pre- sumptive Staphylococcus aureus and were further analyzed by gram's reaction and biochemical tests (catalase test, oxidase test, indole, methyl red, voges-Proskauer, nitrate reduction, coagulase test) andS.aureus was confirmed by the coagulase test. Then samples were examined and antibiogram was per- formed by disc diffusion method on Mueller Hinton agar with 12 antibiotics

Results: We identified 42 coagulase positive Staphylococcus aureus strains from mayonnaise sauce packets. Antibiotic sen- sitivity testing revealed that 40 (95.2%)of isolates were sensi- tive to vancomycin, 36 (85.5%) and 33 (78.5%) were resistant to penicillin and cotrimoxazole, respectively. …

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