Academic journal article Iranian Journal of Public Health

Antibacterial Activity of Four Essential Oil Components on Erwinia Carotovora

Academic journal article Iranian Journal of Public Health

Antibacterial Activity of Four Essential Oil Components on Erwinia Carotovora

Article excerpt

Background: Antibacterial activity of four essential oil com- ponents including thymol, carvacrol, eugenol and menthol has been evaluated towards the vegetable spoilage bacterium, Erwinia carotovora.

Methods: The above mentioned compounds were screened for antibacterial activity using micro broth dilution assay and disc diffusion method against Erwinia carotovora isolated from vegetables. The minimum inhibitory concentration (MIC) values of these components were determined by micro broth dilution assay using a 96-well microtitre plate and MICs were defined as the lowest concentration of the compound that inhibits bacterial growth after 24-48 hours. For the disk dif- fusion method, coated discs were positioned in the center of inoculated agar plate and were incubated at 35±2°C for 24-48 hours; the inhibition zone diameter surrounding the disc was measured in millimeters. Each component was assayed in duplicate.

Results: The MIC value of thymol was the lowest (75 ppm) showing its high potential of bioactivity while eugenol MIC value was the highest (125 ppm) and possessed the weakest effect. …

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