Academic journal article Iranian Journal of Public Health

Prevalence and Antibiotic Susceptibility Pattern of Anaerobic Bacteria Isolated from Surgical Site Infections

Academic journal article Iranian Journal of Public Health

Prevalence and Antibiotic Susceptibility Pattern of Anaerobic Bacteria Isolated from Surgical Site Infections

Article excerpt

Background: The aim of this study was to investigate anaer- obic bacteria prevalence in SSIs and determination of antibi- otic susceptibility pattern in these isolates.

Methods: Between October 2012 and July 2012, 100 surgical site infections obtained by needle aspiration of purulent ma- terial in depth of infected site. These specimens were pro- cessed for detection of anaerobe bacteria in bacteriology lab. For isolation of anaerobic organisms pre-reduced vitamin K enriched Brucella blood agar, Bacteroides Bile Esculin (BBE) agar, kanamycin Vancomycin lacked blood(KVLB) and phe- nyl ethyl alcohol(PEA) agar were inoculated and incubated under anaerobic condition implicated by Anoxomat system at 370 C and examined at 72 and 96h. For antimicrobial drug susceptibility assay in gram negative anaerobic bacteria isolat- ed from these infections, the minimum inhibitory concentra- tion (MIC) of Imipenem, Chloramphenicol, metronidazole, Clindamycin, Cefoxitin, and penicillin G was determined by the agar dilution method. MIC of penicillin, metronidazole, Clindamycin, Cefoxitin for gram positive anaerobic bacteria were determined by E-test strip according to CLSI guideline for anaerobic susceptibility testing. …

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