Academic journal article Iranian Journal of Public Health

Molecular Diagnosis of Shigella Sonnei Isolates with Wbzg Gene in Stool Sample of Children with Diarrhea in Tehran

Academic journal article Iranian Journal of Public Health

Molecular Diagnosis of Shigella Sonnei Isolates with Wbzg Gene in Stool Sample of Children with Diarrhea in Tehran

Article excerpt

Background: The objective of this study was to identify Shigella sonnei among isolates by means of Polymerase Chain Reaction (PCR) with specific gene wbzg and serology method. Shigella spp.wre isolated from stool samples of patients with diarrhea.

Methods: During summer and autumn of 2012, 160 Shigella spp. isolates were collected from children under 10 years old with diarrhea and clinical signs such as fever and cramp and weakness from 2 major hospitals in Tehran, Iran. The pres- ence of ipaH gene was investigated by PCR method with specific pairs of primers and serogrouping of isolates was done by monospecific antisera.Then primer oligonucleotides specific for Shigella sonnei (wbzg) was used for distinguishing one of the most commonly found Shigella species.

Results: Among 160 isolates having the pathogen gene ipaH, 50 isolate were positive for wbgZ gene indicative of Shigella sonnei . …

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