Academic journal article Iranian Journal of Public Health

Intrafamilial Transmission of Helicobacter Pylori: Genotyping of Fecal Samples

Academic journal article Iranian Journal of Public Health

Intrafamilial Transmission of Helicobacter Pylori: Genotyping of Fecal Samples

Article excerpt

Background: Helicobacter pylori (H. pylori) commonly acquired in early childhood. After more than 20 years of research, there is a little information about the details routes of this bacterium transmission. The aim of this study was to explore intrafamilial transmission of H. pylori in children who had indication for upper gastrointestinal endoscopy and their parents, using RAPD-PCR.

Methods: Children (aged up to 15 years) attending Children Medical Center Hospital for upper gastrointestinal endoscopy of suspected H. pylori infection during September 2012 to October 2013 was studied. Then, the parents of those with positive urea breath test (UBT) results were asked to provide fecal and blood samples after informed parental consent. Noninvasive tests such as immunoassay for serological anti- bodies against H. pylori and detection of its antigen in feces were measured. The genetic similarity of the family strains was investigated by amplification of the random amplifica- tion of polymorphic DNA (RAPD-PCR) genotyping meth- od.

Results: According to the genotyping results of 30 families (children with their parents), in 10 (33.3%) children related H. …

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