Academic journal article Iranian Journal of Public Health

Molecular Detection and Speciation of Campylobacter Spp. in Children Gastroenteritis Using Pcr-Rflp Method in Karaj Hospitals

Academic journal article Iranian Journal of Public Health

Molecular Detection and Speciation of Campylobacter Spp. in Children Gastroenteritis Using Pcr-Rflp Method in Karaj Hospitals

Article excerpt

Background: In this survey, PCR-RFLP technique was used for detection and simultaneous speciation of Campylobacter isolates in stool samples and determination of the frequency of Campylobacter contamination in the cases of children gas- troenteritis in Bahonar hospital of Karaj city. To this, two hundred stool samples were obtained from neonates and children up to the age of 8 years with gastrointestinal disor- ders during June to September of 2011.

Methods: DNA of the samples were extracted using DNG plus kit (Cinnagen) and PCR was optimized to amplify a 491 bp fragment of the 23s rRNA gene of Campylobacter genus and C. jejuni RTCC 1097 reference strain as positive control in the clinical samples, then RFLP technique using AluI and TasI was performed to differentiate jejuni, coli, lari and upsa- liensis species

Results: Evaluation of PCR positive samples (21 out of 200/10. …

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