Academic journal article Iranian Journal of Public Health

Occurrence of Putative Virulence Genes in Arcobacter Species Isolated from Animal

Academic journal article Iranian Journal of Public Health

Occurrence of Putative Virulence Genes in Arcobacter Species Isolated from Animal

Article excerpt

Background: The aim of this study was to evaluate the pres- ence of six putative Arcobacter virulence genes (cadF, ciaB, cj1349, mviN, pldA, and tlyA) in A. butzleri, A. cryaerophilus, and A. skirrowii isolates, isolated from food of animal origins obtained from slaughterhouse in Shiraz, Southern Iran, using PCR.

Methods: The isolates (113 A. butzleri, 40 A. cryaerophilus, and 15 A. skirrowii isolates) were confirmed on the basis of poly- merase chain reaction (PCR) amplification of genus and spe- cies specific PCR for determining three species. For con- firmed isolates, PCR was carried out for the presence of viru- lence genes using specific primers.

Results: All A. butzleri isolates carried all six genes. InA. cryaerophilus and A. skirrowii, the cadF gene was detected just in 55 and 53.3%, ciaB in 97.5 and 86.6%, cj1349 in 45 and 60%, mviN in 90 and 80%, pldA in 32.5 and 13.3%, and tlyA in 37.5 and 40%, respectively. InA. cryaerophilus and A. skirrowii, the genes ciaB and mviN were significantly more prevalent than other virulence markers (p <0. …

Search by... Author
Show... All Results Primary Sources Peer-reviewed

Oops!

An unknown error has occurred. Please click the button below to reload the page. If the problem persists, please try again in a little while.