Academic journal article Iranian Journal of Public Health

Homology of Multi Locus Sequence Types of H. Pylori Strains with Different Genotypes in a Single Host

Academic journal article Iranian Journal of Public Health

Homology of Multi Locus Sequence Types of H. Pylori Strains with Different Genotypes in a Single Host

Article excerpt

Background: The purpose of this study was the investiga- tion of multiple sequence types of single isolates of H. pylori presenting diverse genotypes but identical RAPD patterns in a single host by MLST method.

Methods: The gastric biopsy specimens were obtained from the antrum of 98 patients with gastritis and peptic ulcer dis- ease undergoing endoscopy in a gastroenterology unit in Teh- ran. The biopsy specimens were homogenized and cultured. The isolates were identified by PCR for detection of the glmM and also conventional biochemical tests. Six single colonies were isolated from each individual. RAPD-PCR typing (1283 and 1254) and genotyping (vacAs1/s2, va- cAm1/m2, iceA1/iceA2) were performed for each single iso- late. Multi locus sequence typing (MLST) of seven gene loci (atpA, efp, mutY, ppa, ureI, trpC, yphC) were done for strains with identical RAPD patterns but diverse genotypes.

Results: Out of the 98 patients, 39 patients were H. pylori positive (39.8%). Mean age of the subjects was 35 years old. Males and females constituted 36% and 63% of these pa- tients, respectively. Analysis of the obtained RAPD-PCR patterns among the single strains in a host showed identical RAPD patterns in 23% of the infected patients. …

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