Academic journal article Iranian Journal of Public Health

Prevalence of Cdtb of Campylobacter Spp. in Patients with Gasteroenteritis

Academic journal article Iranian Journal of Public Health

Prevalence of Cdtb of Campylobacter Spp. in Patients with Gasteroenteritis

Article excerpt

Background: Our objective was to detect the prevalence of cdtB gene in patients with acute gasteroenteritis.

Methods: This study was conducted on stool samples that obtained from patients with acute gasteroenteritis. Genomic DNA was extracted with the Qiagene (QIAamp DNA Mini Kit) according to the manufacturés instruction. Detection of cdtB was determined by PCR using specific primers. The sus- pected PCR products were subjected to sequencing by using an automated sequencer (source Bioscience, UK). Presence of Campylobacter spp. DNA in positive samples was also de- tected by PCR with genus specific primers.

Results: We assessed a total of 50 stool samples. The PCR results showed that 32% of the samples were positive for cdtB. Also the PCR reaction for Campylobacter genus was posi- tive in these samples. These results were finally confirmed by sequencing.

Conclusion: The results of this study showed high preva- lence of Campylobacter spp. among patients with gastritis. …

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