Academic journal article Iranian Journal of Public Health

The Prevalence of Mycoplasma Agalactiae in Sheep Herds with and without Sings of Contagious Agalactia in Kurdistan Province

Academic journal article Iranian Journal of Public Health

The Prevalence of Mycoplasma Agalactiae in Sheep Herds with and without Sings of Contagious Agalactia in Kurdistan Province

Article excerpt

Background: The aim of the present study was to detect Mycoplasma species and M. agalactiae in conjunctival, synovial fluid, nasal, ear and milk samples in sheep herds with or without CA sign in Kurdistan province by PCR.

Methods: Between 2012 and 2013, one hundred and seventy three samples analyzed were taken from sheep herds reared in a CA endemic area with (113 samples) and without (60 samples) signs of CA syndrome. The PCR with Mycoplasma 16S rRNA was applied for detection of a variety of Mycoplas- ma species. In this study two primers (forward and reverse) amplify 163bp region of 16S rRNA gene of Mycoplasma genus and amplify 375bp region of 16S rRNA gene of M. agalactiae species were used.

Results: A total number of 113 samples were analyzed, of which 105 (93.0%) proved positive for the presence of Myco- plasma species in sheep with signs of CA syndrome. From these positive samples, 13 isolates (12.4%) was positive for M. agalactiae. M. agalactiae was detected in conjunctival (4/50), synovial fluid (1/7), and milk (8/17) samples. …

Search by... Author
Show... All Results Primary Sources Peer-reviewed

Oops!

An unknown error has occurred. Please click the button below to reload the page. If the problem persists, please try again in a little while.