Academic journal article Iranian Journal of Public Health

Rapid Identification of Clinically Relevant Nocardia Spp. by 16s Rrna Gene Pcr in Aids Patients

Academic journal article Iranian Journal of Public Health

Rapid Identification of Clinically Relevant Nocardia Spp. by 16s Rrna Gene Pcr in Aids Patients

Article excerpt

Background: In this study, we developed an alternative PCR-based identification strategy targeted at the gene coding for 16S rRNA. Although the sequence for this gene is largely phylogenetically conserved, there may be variable sequences characteristic of particular organisms. The detection of these variable regions can therefore allow bacteria to be identified and differentiated from each other.

Methods: Primers NG1 and NG2 were used to amplify a Nocardia genus-specific 595-bp fragment of 16S rRNA. PCR test was optimized by Nocardia brasiliensis standard strain. Limit of detection and specificity test evaluated and then 30 AIDS patient serum samples was extracted by DNG kit. Amplicon was cloned in E. coli JM107 by pTZ57R plasmid and sequenced by Dideoxy chain termination.

Results: PCR testing was performed on 30 samples and 595- bp amplified product was observed by agarose gel electro- phoresis. 2(6.6%) of 30 samples were positive for Nocardia PCR. …

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