Academic journal article Iranian Journal of Public Health

Hbv Infection and Il-27 Gene Expression in Liver Transplant Patients

Academic journal article Iranian Journal of Public Health

Hbv Infection and Il-27 Gene Expression in Liver Transplant Patients

Article excerpt

Background: In this study the possible association between IL-27 expression level in HBV-infected liver transplant pa- tients was evaluated.

Methods: In a cross sectional study, 50 liver transplant pa- tients sub grouped to each 25 HBV infected and non- infected ones were enrolled between years.The 25 healthy controls were also evaluated. Screening and sub grouping patients to HBV infected and non-infected ones were ana- lyzed using immunologic and molecular diagnostic meth- ods.The expression of IL-27 gene was evaluated in three studied groups using in-house Syber Green Real Time PCR method: The GAPDH gene was used as internal control for Real-Time PCR reactions. The rate of increased expression was calculated using the Livak(2-ΔΔCT) method.

Results: The expression level of IL-27 gene was significantly higher in HBV infected liver transplant patients compared with non-infected ones. The expression level of IL-27 gene was also significantly higher in HBV infected liver transplant patients compared with healthy controls. …

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