Academic journal article Iranian Journal of Public Health

MOLECULAR CHARACTERISTICS OF HEPATITIS B VIRUS ISOLATED FROM INTRAVENOUS DRUG USERS IN TEHRAN-Iran, 2013

Academic journal article Iranian Journal of Public Health

MOLECULAR CHARACTERISTICS OF HEPATITIS B VIRUS ISOLATED FROM INTRAVENOUS DRUG USERS IN TEHRAN-Iran, 2013

Article excerpt

Background: We investigated the possible genotypes in HBV-infected IVDU in Tehran.

Methods: This study was conducted on 229 intravenous drug users who referred to three Drop- in-Centers and Loghman hospital in Tehran. Their serum samples were test- ed for the presence of HBsAg (Hepatitis B surface Antigen) by Enzyme-Linked Immunosorbent Assay (ELISA). Then HBV DNA was extracted from HBsAg positive samples and a fragment of the S gene amplified by Nested PCR. HBV genotype, subgenotypes were determined by direct sequenc- ing.

Results: Out of 229 individuals, 5 (2.1%) subjects were found to be serological positive for HBsAg, and HBV DNA was found in 3 HBsAg positive cases. Phylogenetic tree drawn by neighbor- joining method in HBV DNA positive drug users confirmed the existence of genotype D (subgeno- type D1/ D2, subtype ayw2).

Conclusion: Classifying HBV into genotypes has to be cost- effective and clinically relevant, specially in high risk groups such as intravenous drug users. The most important finding of our study was that the only HBV genotype D was detected in all patients; our study concurs with other reports from Iran, all showing that genotype D is the only detectable geno- type in Iran up to now. …

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