Academic journal article Iranian Journal of Public Health

Designing of Elisa Kit for Rapid Detection of Mycobacterium Tuberculosis Infection with Recombinant Cfp10 Protein

Academic journal article Iranian Journal of Public Health

Designing of Elisa Kit for Rapid Detection of Mycobacterium Tuberculosis Infection with Recombinant Cfp10 Protein

Article excerpt

Background: In the present study using Indirect ELISA, we evaluated IgG class antibodies among tuberculosis patients against recombinant CFP10 antigen.

Methods: In this study, Indirect ELISA method was used to investigate the serum reactivity to CFP10 antigen in 4 patient groups. Seventy serum samples of smear, culture and/or PCR positive cases, 5 serum samples from patients who have infections with non-tuberculosis mycobacteria, 20 cases of smear, culture and PCR negative, and 46 serum samples from healthy individuals (as negative control) were studied for IgG antibody levels during one year study (91-92) in mycobacteri- ology department of Pasteur institute of Iran.

Results: On average, recombinant antigen CFP10, reacts with 90% of all serum samples from patients with tuberculo- sis, by ELISA method. This issue shows accepted sensitivity of this antigen, especially for serum samples from tuberculo- sis cases with TB culture, smear and PCR positive (100% sensitivity). …

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