Academic journal article Iranian Journal of Public Health

Analysis of Single Nucleotide Polymorphisms (Snps) in Pertussis Toxin Promoter of Vaccine and Clinical Isolates of Bordetella Pertussis

Academic journal article Iranian Journal of Public Health

Analysis of Single Nucleotide Polymorphisms (Snps) in Pertussis Toxin Promoter of Vaccine and Clinical Isolates of Bordetella Pertussis

Article excerpt

Background: The aim of this study is to identify the single nucleotide polymorphisms (SNPs) of pertussis toxin promot- er (ptxP) in clinical isolates and vaccine strains.

Methods: Five clinical isolates of Bp, Tohama I as the refer- ence strain, and 2 vaccine strains employed in Iran were used in this study. All of the isolates and strains were confirmed to be Bp by phenotypic and molecular tests. ptxP gene was am- plified by PCR, cloned in TA vector and sequenced. The sequences were aligned and analyzed via Bio Edit and Clus- talX.

Results: Three distinct ptxP alleles were identified. The clin- ical isolates displayed the ptxP3 allele, whereas both vaccine strains and Tohama I was found to have ptxP1 and ptxP2 alleles, respectively.

Conclusion: In contrast to the vaccine strains, Bp strains presently circulating in Iran have the ptxP3 allele type. This change is a global phenomenon, which seems to be associat- ed with resurgence of pertussis. The ptxP SNPs are mostly located in a region involved in regulation of transcription. This region is involved in binding to BvgA gene, which regu- lates the expression of the virulence genes in Bp. …

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