Academic journal article Iranian Journal of Public Health

Evaluation of Humoral Immune Response in Tuberculosis Patients Than Recombinant Tb10.4 Antigen by Elisa Method

Academic journal article Iranian Journal of Public Health

Evaluation of Humoral Immune Response in Tuberculosis Patients Than Recombinant Tb10.4 Antigen by Elisa Method

Article excerpt

Background: The aim of this study was to determine the antibody level measurement among patients with tuberculosis in comparison with the control group against recombinant TB10.4 antigen by ELISA method.

Methods: Sixty eight serum samples from TB suspicious patients were referred to the Pasteur Institute during 2 years (91-92), 44 serum samples from healthy individuals and 5 samples from individuals infected with atypical mycobacteria were collected. In order to assess IgG class antibodies against recombinant TB10.4 antigen, Indirect ELISA method was applied. After optimization of all components, data analyzed in comparison with the absorbance of negative control and the range of results interpretation was determined.

Results: The recombinant TB10.4 antigen, on average with 96% of all serum samples from TB patients showed a posi- tive reaction which shows sensitivity over 100%. On the oth- er hand, among negative control serum samples with a histo- ry of BCG vaccination or isolation of environmental Mycobac- teria also positive results were observed. …

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