Academic journal article Iranian Journal of Public Health

Prepartion of a Gene Construct from Protective Domains of Leishmania Major

Academic journal article Iranian Journal of Public Health

Prepartion of a Gene Construct from Protective Domains of Leishmania Major

Article excerpt

Background: Leishmaniasis is an infectious disease affecting millions of people worldwide. The treatment of the disease is hampered due to high cost, toxicity and the crisis of drug resistance. Polytope approaches of genetic immunization could be a strategy for prevention of infectious diseases. Fur- thermore, the identification of leishmania genome sequence and the application of bioinformatics assist us to devise an effective vaccine's candidate.

Methods: In current study, we have designed a linear se- quence from three predicted epitopes of GP63, LACK and CPC antigens by "T-cell prediction" server. The synthesized sequence regarded as "LAKJB93" was ligated to pEGFP-N1 plasmid. In current study, we have designed a linear sequence from three predicted epitopes of GP63, LACK and CPC antigens by "T-cell prediction" server. The synthesized se- quence regarded as "LAKJB93" was ligated to pEGFP-N1 plasmid. In current study, we have designed a linear sequence from three predicted epitopes of GP63, LACK and CPC antigens by "T-cell prediction" server. The synthesized se- quence regarded as "LAKJB93" was ligated to pEGFP-N1 plasmid. …

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