Academic journal article Iranian Journal of Public Health

MOLECULAR ANALYSIS OF THE LIPL41 GENE WITHIN LEPTOSPIRA INTERROGANS STANDARD SEROVARS IN Iran

Academic journal article Iranian Journal of Public Health

MOLECULAR ANALYSIS OF THE LIPL41 GENE WITHIN LEPTOSPIRA INTERROGANS STANDARD SEROVARS IN Iran

Article excerpt

Background: In order to homological (polymorphism) analysis we sequenced and compared lipl41 genes cloned from standard pathogenic serovars of Leptospira prevalent in Iran.

Methods: Eleven pathogenic serovars and one saprophytic species were inoculated into the selective culture medium EMJH and the genomic DNA was extracted. The lipl41 gene was amplified by specific primers and cloned into a pTZ57R/T vector and transformed in competent E. coli Top10 cells. The extracted recombinant plasmid were se- quenced. The percentage identity and divergence among dif- ferent leptospiral serovars was deduced using the Blast and MegAlign program.

Results: PCR amplification of the lipl41 gene resulted in a 1065bp lipl41 gene product in all eleven pathogenic serovars tested. No PCR products were amplified from the non- pathogenic L. …

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