Academic journal article Iranian Journal of Public Health

Detection of Maga Gene among Esbl-Positive and Esbl-Negative Klebsiella Pneumoniae Isolated from Clinical Samples

Academic journal article Iranian Journal of Public Health

Detection of Maga Gene among Esbl-Positive and Esbl-Negative Klebsiella Pneumoniae Isolated from Clinical Samples

Article excerpt

Background: The present study was performed to deter- mine the prevalence of magA gene among extended- spectrum betalactamase (ESBL)-positive and ESBL-negative K. pneumoniae.

Methods: The current cross-sectional study was conducted on 130 K. pneumoniae isolates collected from patients at Imam Reza hospital and its associated clinics in the city of Mashhad (Iran) from May 2011 to July 2012. The presence of K. pneu- moniae species were confirmed by conventional microbiologi- cal methods. Samples were tested for production of ESBLs by double disk diffusion (DDS) test. PCR was performed to detect magA gene. The hypermucoviscosity (HV) phenotype of Klebsiella isolates was characterized by string test.

Results: MagA gene was detected in 11(8.5%) isolates of K. pneumoniae. Of 11 isolates with positive result for magA gene, three were HV + and 8 were HV - phenotype. …

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