Academic journal article Iranian Journal of Public Health

Inhibitory Effect of Lactobacillus Reuteri on Pathogenic Bacteria Isolated from Women with Bacterial Vaginosis

Academic journal article Iranian Journal of Public Health

Inhibitory Effect of Lactobacillus Reuteri on Pathogenic Bacteria Isolated from Women with Bacterial Vaginosis

Article excerpt

Background: The purpose of this study was to investigate the inhibitory effect of Lactobacillus reuteri on pathogenic bac- teria isolated from women with bacterial vaginosis, respec- tively.

Methods: 96 samples from women with bacterial vaginosis discharge were taken by a gynecologist with a dacron swab and put in sterile tubes containing TSB broth and Thioglycol- late broth and were immediately sent to the lab location in cold chain for the next stages of investigation. From Thiogly- collate and TSB medium was cultured on blood agar and EMB and Palkam and Differential diagnosis environments. Then incubated for 24 h at 37 ° C. Strains of Lactobacillus reuteri were cultured in MRSA environment and were trans- fered to the lab. After purification of pathogenic bacteria, MIC methods and antibiogram, Lactobacillus reuteri inhibitory effect on pathogenic bacteria is checked. Statistical analysis was done by SPSS software v.16.

Results: The results of this study demonstrate the inhibitory effect of Lactobacillus reuteri on some pathogenic bacteria that cause bacterial vaginosis, including Staphylococcus aureus, Staphy- lococcus epidermidis, Staphylococcus saprophyticus, Streptococcus agalac- tiae, Entrococcus, Listeria monocytogenes and E. …

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