Academic journal article Iranian Journal of Public Health

Occurrence of Integrons and Antimicrobial Resistance Genes among Clinical Isolates of Enterobacter Spp. from Hospitals of Tehran

Academic journal article Iranian Journal of Public Health

Occurrence of Integrons and Antimicrobial Resistance Genes among Clinical Isolates of Enterobacter Spp. from Hospitals of Tehran

Article excerpt

Background: The objectives of this study were to determine the prevalence of three classes of integrons in isolated Entero- bacter spp. and to find out the association between the pres- ence of integrons and antibiotic resistance.

Methods: A total of 110 clinical isolates of Enterobacter were isolated from several hospitals in Tehran between 2012 to 2013. Enterobacter species were identified by using API 20E system. The existence of integron classes was investigated by PCR assay through the amplification of integrase genes. In isolates with one of the integrons, antibacterial susceptibility the fourteen antibiotic disks were determined by disk diffu- sion and ESBL phenotype was confirmed by Combined Disk. Then, the bla groups, blaTEM, blaSHV, blaCTX-M-1 and aminoglycoside modifying enzymes genes were identified by PCR with specific primers.

Results: The prevalence of Enterobacter species were E. cloacae (78.2 %), E. aerogenes (13.6 %) and E. sakazakii (8.2%). They were from different clinical sources. Forty four of En- terobacter isolates have integron but there was not detected class 3 of integrons among isolates. …

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