Academic journal article Iranian Journal of Public Health

Prevalence of Malassezia Species Isolated from Skin of Patients with Seborrheic Dermatitis Reffered to Tonekabon Clinics by Pcr- Sequencing Method

Academic journal article Iranian Journal of Public Health

Prevalence of Malassezia Species Isolated from Skin of Patients with Seborrheic Dermatitis Reffered to Tonekabon Clinics by Pcr- Sequencing Method

Article excerpt

Background : In this study, the yeast were studied by using real-time PCR method for the samples of skin scales from patients with seborrheic dermatitis and dandruff, skin clinic Tonekabon city

Methods: In this study, 65 collected from behind the ears, scalp, nails,dandruff are transferred onto selective media (SDA,Mdixon) to avoid dehydration of the yeasts After per- forming diagnostic tests to determine the biochemical and fungal species.Genomic DNA extraction by phenol- Chloroform method was done.for real-time PCR,ITS1-4 uni- versal primers were used. PCR products for identification of Malassezia were sequenced

Results: Isolates obtained by cultured and biochemical test included: 4 case Malassezia globosa, 2case Malassezia restricta. Three colonies of fungus were not identified by culture and biochemical test. Cultural and biochemical identification was confirmed by molecular test. …

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