Academic journal article Iranian Journal of Public Health

Determine and and Prevalence of Ctx-M and Ctx-M-15 in Klebsiella Pneumonia Isolates from Clinical Cases of Kerman

Academic journal article Iranian Journal of Public Health

Determine and and Prevalence of Ctx-M and Ctx-M-15 in Klebsiella Pneumonia Isolates from Clinical Cases of Kerman

Article excerpt

Background: The purposes of this study were to determine the presence and prevalence of CTX-M and CTX-M-15 genes in Klebsiella pneumoniae isolates from clinical cases of Kerman

Methods: One hundred thirty two clinical samples were ob- tained during six month from different parts of Kerman hos- pitals and for identification of Klebsiella pneumoniae were re- ferred to laboratory of veterinary faculty of shaheid Bahonar university of Kerman. Samples were cultured for isolation of Klebsiella pneumoniae. The suspected isolates were confirmed according to the biochemical tests. DNA was extracted from all of the confirmed isolates and reference strains by lysis method. PCR assay was used for detection of mentioned genes.

Results: Among the examined samples 92 Klebsiella pneumoni- ae were confirmed, which least 20 isolate was possessed one of the examined genes. …

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