Academic journal article Iranian Journal of Public Health

Evaluation of Induced Antibodies by Microparticles Containing Conjugated Detoxified Lps from Salmonella Paratyphi C to Tetanus Toxoid

Academic journal article Iranian Journal of Public Health

Evaluation of Induced Antibodies by Microparticles Containing Conjugated Detoxified Lps from Salmonella Paratyphi C to Tetanus Toxoid

Article excerpt

Background: The purpose of this study is to design new candidate vaccine and assess its immunogenicity against Sal- monella paratyphi C.

Methods: LPS extracted by hot phenol procedure and LPS Conjugation to TT was performed using amidation proce- dure, and for purification conjugated molecules column chromatography with 2B-Cl have been used and microparti- cles was prepared by emulsion technique in iso-octane. To evaluate the immunogenicity of prepared antigens, purified LPS, pure tetanus toxoid, normal saline and LPS-TT micro- particles either injected intraperitoneally with a final volume of 0/5 mg to female mice BALB/c (6-8 weeks), and three times injection was conducted at two-week intervals and heart blood of mice was performed two weeks after injection. Collected serum stored at a temperature of 70 °C and specific lipopolysaccharide antibodies titers were determined by indi- rect ELISA.

Results: In this study mice IgM, IgG, IgA and IgG1, IgG2a, IgG2b and IgG3 subclasses against LPS of Salmonella para- typhi C were determined. …

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