Academic journal article Iranian Journal of Public Health

Preparation of Detoxified Loaded Alginate Microparticles Lps Salmonella Paratyphi C with Tetanus Toxoid

Academic journal article Iranian Journal of Public Health

Preparation of Detoxified Loaded Alginate Microparticles Lps Salmonella Paratyphi C with Tetanus Toxoid

Article excerpt

Background: The goal of this research is to design vaccine against Salmonella paratyphi C.

Methods: LPS extraction was performed using phenol-water and LPS Conjugation to the TT was applied by amidation method. For purification of conjugated molecules Sepharose Cl-2B column chromatography was used. Collected fractions at two wavelengths 210 and 280 nm were read out and tubes with the highest absorbance at both wavelengths have been collected and are being merged. Then the alginate microparti- cles were prepared by emulsification in the iso-octane organic phase.

Results: The results of SDS-PAGE electrophoresis contain- ing urea with 4M silver staining was shown that healthy LPS were isolated from bacteria. Fractions 83 and 84 have highest absorb by both wavelengths of 210 and 280 and have merged and after alginate microparticles were prepared and photo- graphed by light microscopy, which showed the formation of solid microparticles and particle size analyzer was used to measure the diameter of microparticles that the diameter of the particle was 89. …

Search by... Author
Show... All Results Primary Sources Peer-reviewed

Oops!

An unknown error has occurred. Please click the button below to reload the page. If the problem persists, please try again in a little while.