Academic journal article Iranian Journal of Public Health

Isolation and Molecular Identification of Basillus Pumilus from Wound Infection for the First Time in Iran

Academic journal article Iranian Journal of Public Health

Isolation and Molecular Identification of Basillus Pumilus from Wound Infection for the First Time in Iran

Article excerpt

Background: Bacillus pumilus is used for transferring genetic materials and its infecting cases have been rarely reported among humans. In human, it mostly induces secondary infec- tion.

Methods: In October 2012 a male patient with a sore back at the age of 41 years was sampled by sterile swab. Then the wound specimens were cultured on Blood Agar medium. After incubation, the culture medium put at 37 ° C in aerobic conditions and 5% CO2. PCR was used to determine the molecular identity.

Results: After various biochemical tests, PCR, and sequenc- ing of the 16S rRNA gene analysis showed 99% homology with the target gene based on the gene sequence. It was a strain of Bacillus pumilus. Target gene in the NCBI GeneBank database access numbers were AB894358. This is the first report of isolation of Bacillus pumilus of bedsores in Iran. …

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