Academic journal article Iranian Journal of Public Health

Spread of Multidrug-Resistant Acinetobacter Baumannii Isolates in Icu Patients

Academic journal article Iranian Journal of Public Health

Spread of Multidrug-Resistant Acinetobacter Baumannii Isolates in Icu Patients

Article excerpt

Background: The aims of this study were to evaluate the OXA-type carbapenemase genes in clinical isolates of A. baumannii and investigate genetic relationship among the iso- lates.

Methods: One hundred and twenty-five clinical isolates of Acinetobacter baumannii were obtained from hospitalized pa- tients from June to Desember 2010 in Imam Hossein Hospi- tal, Tehran. Isolates were identified by conventional bio- chemical tests such as oxidase test, motility, growth at 44°C and then were confirmed by amplification of blaoxa-51-like gene using PCR. Susceptibility to antimicrobial agents was assessed by the disk diffusion method. DNA extraction was carried out by boiling method. The presence of OXA-type carbapenemase genes detected by Multiplex PCR. Molecular typing of isolates was performed using repetitive extragenic palindromic sequence based polymerase chain reaction (REP- PCR) method. Then, REP-PCR fingerprints were analysed with GelCompar II software.

Results: Our results showed that A. baumannii strains were isolated more frequently from patients hospitalized in ICU (74.4%) other than wards and the most rate of isolation was from respiratory secretions (53. …

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