Academic journal article Iranian Journal of Public Health

The Prevalence of Bacterial Infections in Children Undergoing Dialysis with Culture Method and the Pcr, and Determination Drug Sensitivity and Risk Factors of Infection

Academic journal article Iranian Journal of Public Health

The Prevalence of Bacterial Infections in Children Undergoing Dialysis with Culture Method and the Pcr, and Determination Drug Sensitivity and Risk Factors of Infection

Article excerpt

Background: Diagnosis of infection caused by bacteria and determining of their antibiotic resistance patterns may be helpful in treatment of patients and increasing of life quality.

Methods: Blood or peritoneum samples of hospitalized children in dialysis ward of Bahrami, Hazrat Ali Asghar and Mofid hospitals with clinical symptoms of peritonitis in peritoneal dialysis or septicemia in the hemodialysis were collected and sent to the laboratory. Microbiological and biochemical tests revealed bacterial infection in 4 samples of 42 and sensitivity test performed for all 4 bacterial isolates. PCR performed for 4 isolates using specific primers and PCR products were electrophoresed in 1.5% agarose gel containing ethidium bromide. DNA ladder was used to detect the molecular weights of observed bands under UV lamp. Finally, results of microbiological and molecular methods were compared.

Results: The isolated bacteria were E.coli, Klebsiella pneumoniae, P.aeruginosa and Acinetobacter baumannii. All of them were susceptible to Gentamycin and Ciprofloxacin and resistant to the Ampicillin. As expected, all positive cultures had positive reaction in PCR. None of the negative cultures had band in agarose gel after PCR. …

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