Academic journal article Iranian Journal of Public Health

Evaluation of Humoral Immunity of Haemophilus Influenzae Type B Prp Polysaccharid Conjugate with Tetanus Toxoid

Academic journal article Iranian Journal of Public Health

Evaluation of Humoral Immunity of Haemophilus Influenzae Type B Prp Polysaccharid Conjugate with Tetanus Toxoid

Article excerpt

Background: Our aim in this study was to increase im- munugenicity of PRP by conjugating PRP to Tetanus toxoid.

Methods: Haemophilus influenza type B PTCC1623 was culti- vated in 10L of CY broth medium in fermentor, and purified by alcohol precipitation method, and by the addition of Ce- tavlon, and Hydroxyl Apatite. Tetanus toxoid was provided by Razi Vaccine & Serum Institute. 5mg of PRP was conju- gated to 2 mg of tetanus toxoid by chu method. In this method, Adipic Acid Di Hydrazide (ADH) and Cyanogen bromide were used as the spacer, and 1-Ethyl-3,3-di Methyl Amino Propyl (Carbo Di Amid) (EDAC) was used as the linker. The conjugate obtained was purified by flowing through Chromatography Column, using 4B-CL gel. Immun- ization was done choosing1 2 groups of white New Zealand rabbits. 25µg of pure PRP in 0. 5ml serum physiology and 0.5 of the conjugate were injected to groups 1 and 2, with a 15 day interval, intramuscularly, respectively. …

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