Academic journal article Iranian Journal of Public Health

Extended-Spectrum Beta-Lactamase Producing Klebsiella Pneumoniae Isolates from Patients with Cystic Fibrosis

Academic journal article Iranian Journal of Public Health

Extended-Spectrum Beta-Lactamase Producing Klebsiella Pneumoniae Isolates from Patients with Cystic Fibrosis

Article excerpt

Background: The aim of this research was to determine the antibiotic resistance profiles as well as the prevalence of β- lactamase production in K. pneumoniae isolates from CF pa- tients in Tehran.

Methods: A total of 16 non-repetitive K. pneumoniae strains were isolated from 98 children with cystic fibrosis at Mofid Children's Hospital in Tehran between July 2012 and Febru- ary 2013. Bacterial isolates were identified by standard bio- chemical tests. Antimicrobial susceptibility of the isolates was determined to 19 antibiotics by disc diffusion and minimum inhibitory concentrations (MIC) for ceftazidime were meas- ured by microdilution. ESBL production was detected by the conventional double disc synergy test (DDST) as recom- mended by the CLSI. Presence of TEM, SHV, CTX-M, and OXA type β-lactamase genes was shown using specific pri- mers and PCR. Sequence analysis of PCR all products was performed using lasergene software version 6.

Results: Antibiotic resistance rates were: ticarcillin (93.8%), cefotaxime and carbenicillin (75%), ceftazidime, ceftriaxone and piperacilin (68.8%), aztreonam and cotrimoxazole (62.5%), tobramycin (50%), cefepime and co-amoxiclave (37.5%), amikacin and ampicillin-sulbactam (31.3%), cefox- itin and ciprofloxacin (18.8%) and ertapenem (6. …

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