Academic journal article Iranian Journal of Public Health

Microbiology of Diabetic Foot Infections

Academic journal article Iranian Journal of Public Health

Microbiology of Diabetic Foot Infections

Article excerpt

Background: The aim of this research is bacteriological evaluation of diabetic foot ulcer infections, which is essential to prevent worsening, and deterioration of these lesions.

Methods: Between October 2013 and July 2013, 30 diabetic foot infection specimens obtained by needle aspiration of purulent material in depth of infected foot. Chocolate, sheep blood, phenyl ethyl alcohol and MacConkey agar plate were inoculated for isolation of aerobic organisms. These plates incubated at 370 C under 10% CO2 and examined at 24 and 48h. For isolation of anaerobic organisms pre-reduced vita- min K enriched Brucella blood agar, bacteroides bile esculin (BBE) agar, kanamycin vancomycin laked blood(KVLB) and phenyl ethyl alcohol(PEA) agar were inoculated and incubat- ed under anaerobic condition implicated by Anoxomat sys- tem at 370 C and examined at 72 and 96h. All isolates were later identified by biochemical tests.

Results: Altogether a total of 37 aerobic and anaerobic bac- teria isolated from 30 specimens. Predominant aerobic bacte- ria isolated from these infections were Staphy-lococcusaureus (24. …

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