Academic journal article Iranian Journal of Public Health

The Study of Antimicrobial and Anti-Adhesive Effect of Probiotic Lactobacilli on Uropathogenic Escherichia Coli (Upec)

Academic journal article Iranian Journal of Public Health

The Study of Antimicrobial and Anti-Adhesive Effect of Probiotic Lactobacilli on Uropathogenic Escherichia Coli (Upec)

Article excerpt

Background: The aim of this study was to investigate the antimicrobial and especially anti adhesive characteristics of probiotic bacteria against Escherichia coli by using microbial techniques.

Methods: In this study two strains of Lactobacillus acidophi- lus PTCC 1643 and Lactobacillus casei PTCC 1608 have been used to investigate the antimicrobial and anti -adhesive ef- fects on 20 selected samples from total of 40 UPEC collected from Semnan province hospitals. To evaluate the antimicro- bial activity of complete culture and supernatant of probiotic lactobacilli, modified double layer method and dilution of supernatant were used, respectively. The mechanism of co- aggregation of lactobacilli with pathogens was also examined. The microtitre plate method was used to detect anti adhesion activity of Lactobacilli supernatant. …

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