Academic journal article Iranian Journal of Public Health

Survey of Microbial Contamination Prevalance and the Effective Factors in Ilam University of Medical Sciences Student's Toothbrushes

Academic journal article Iranian Journal of Public Health

Survey of Microbial Contamination Prevalance and the Effective Factors in Ilam University of Medical Sciences Student's Toothbrushes

Article excerpt

Background: The most common method of oral hygiene is tooth brushing. However, occasionally brushing becomes a risk factor for health. The main reason is getting infected with various microorganisms. The purpose of this study was to investigate bacterial and fungal contamination of tooth- brushes students, and factors affecting the contamination

Methods: Ninety five brushes were assessed in this study. Samples were incubated in test tubes containing nutrient broth for twenty-four hours. Then the microorganisms were isolated using specific nutrient medium containing Blood agar, Chocolate agar MacConkey and Sabouraud Dextrose agar. The inoculated plates were incubated for 24 to 48 hours at 37 C. The identification of micro-organisms was per- formed based on biochemical tests.

Results: The infection rate was more than 40% of the sub- ject staphylococcus, Niesseria, Diphteroids, Actinomycetes and Enterobacteriacae family members were isolated from samples. Microbial contamination was higher in women than men (P<0.05).There was found correlation between the number of microorganism on brush with time of use, using mouthwash and gender (P<0.05). There was statistically sig- nificant relation between cocci microorganisms' infections with sexes as well as between Bacillus infections with capped brush. …

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