Academic journal article Iranian Journal of Public Health

Effect of Efflux Pump Inhibitor Phenylalanine- Arginine B-Naphthylamide on the Minimum Inhibitory Concentration of Imipenem in Mdr Acinetobacter Baumannii Isolated from Burnt Patients

Academic journal article Iranian Journal of Public Health

Effect of Efflux Pump Inhibitor Phenylalanine- Arginine B-Naphthylamide on the Minimum Inhibitory Concentration of Imipenem in Mdr Acinetobacter Baumannii Isolated from Burnt Patients

Article excerpt

Background: Efflux pumps of the RND super family such as AdeABC play a significant role in producing multidrug resistance A.baumannii as important agents in burn patients. So, the aims of this study were determination the role of ef- flux pump, effect of β -naphthylamide (PaβN) and also the frequency of adeABC genes in imipenem resistance of A. baumannii strains isolated from burnt patients.

Methods: This study was conducted on 60 A. baumannii iso- lates collected from 240 wound samples of burnt patients admitted to the Shahid Motahari Hospital of Tehran during 2013. Isolates identified by using standard methods, Micro- gen kit and existence of OXA-51gene. Antibiotic susceptibil- ity tests were performed by Kirby-Bauer disc diffusion and MIC by broth microdilution methods according to CLSI 2013. The DNA purification was done by the phenol- chloroform method.Activity of the efflux pumpwas evaluated by using efflux pump inhibitor(PaβN).The frequency of ade- ABC genes were detected by PCR and sequencing methods.

Results: Of all isolates, 100% were resistant to imipenem. …

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