Academic journal article Iranian Journal of Public Health

Effect of Recombinant Neutrophil-Activating Protein (Hp-Nap) of Helicobacter Pylori on Peritoneal Macrophages

Academic journal article Iranian Journal of Public Health

Effect of Recombinant Neutrophil-Activating Protein (Hp-Nap) of Helicobacter Pylori on Peritoneal Macrophages

Article excerpt

Background: The aim is effect of recombinant neutrophil- activating protein (HP-NAP) of helicobacter pylori on peritoneal macrophages (MQ).

Methods: Recombinant neutrophil-activating protein (HP- NAP) of helicobacter pylori were produced and in different con- centration (0.01-100µg/ml) affected on macrophage lipopol- ysaccharide (LPS=10µg/ml) activated MQ; then proliferation assayed by MTT method. Nitrite levels were measured by using the diazotizationmethod based on the Griess reaction and MTT assay for evaluation of macrophages.

Results: Proliferation of Macrophages in all treated groups was activated. In LPS stimulated groups in dose dependent manner in high doses (10 and100?g/ml). Macrophages viabil- ity in all groups was the same and NO activation in unstimu- lated groups in all doses and in LPS stimulated groups in 3 higher doses (1,10 and100? …

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