Academic journal article Iranian Journal of Public Health

PHENOTYPIC CHARACTERIZATION OF UROPATHOGENIC ESCHERICHIA COLI STRAINS ISOLATED FROM PATIENTS WITH URINARY TRACT INFECTION IN TEHRAN, Iran

Academic journal article Iranian Journal of Public Health

PHENOTYPIC CHARACTERIZATION OF UROPATHOGENIC ESCHERICHIA COLI STRAINS ISOLATED FROM PATIENTS WITH URINARY TRACT INFECTION IN TEHRAN, Iran

Article excerpt

Background: In this study we assessed the biofilm formation, heamagglutination and hemolysis in UPEC strains isolated from patients with UTI in Tehran, Iran.

Methods: Biofilm production was assessed with microtiter plate method. In this method, bacteria were added to 96-well microtiter plate for 48 hours, and then the wells were washed and stained with crystal violet dye and biofilms were quantified at OD570 nm after solubilization in acetic acid. The heamolysin production was evaluated on blood agar medium. For Haemagglutination assay, cell pellets were mixed with 3% suspension of guinea pig erythrocytes in the presence or absence of mannose. Rapid clumping of the E. coli in the absence of mannose indicates the presence of type 1 fimbriae.

Results: Of the 103 UPEC isolated, 74 (71.8%) strains displayed a biofilm positive phenotype that 24 (32.4%) of the strains were classified as highly positive, 14 (19%) as moderate positive and 36 (48.6%) as weakly positive. …

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