Academic journal article Iranian Journal of Public Health

Molecular Evaluation of Methicillin Resistant Staphylococcus Aureus (Mrsa) by Pcr to Bacteriological Methods

Academic journal article Iranian Journal of Public Health

Molecular Evaluation of Methicillin Resistant Staphylococcus Aureus (Mrsa) by Pcr to Bacteriological Methods

Article excerpt

Background: The objective of the study was to evaluate the efficacy of cefoxitin disc diffusion method to detect MRSA and compare it with other phenotypic methods such as oxacillin disc diffusion test, oxacillin agar screening test and gold standard molecular method, which, as of now, is by the detection of mecA gene by polymerase chain reaction (PCR).

Methods: The study included75 Staphylococcus aureus (S. aureus) isolates obtained from clinical samples. All isolates were tested for MRSA using oxacillin screen agar plates with 4% NaCl and 6 Tg/ml of oxacillin, cefoxitin disk diffusion method using 30 Tg disc and zone sizes were measured and interpreted as per standard guidelines. Amplification of the mecA gene was performed by PCR.

Results: Out of 75 isolates, MRSA was identified in 45. …

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