Academic journal article Iranian Journal of Public Health

The Prevalence of Escherichia Coli Strains Carrying Virulence Gene Ipah Isolated from Different Water Sources in Alborz Province

Academic journal article Iranian Journal of Public Health

The Prevalence of Escherichia Coli Strains Carrying Virulence Gene Ipah Isolated from Different Water Sources in Alborz Province

Article excerpt

Background: E. coli is considered as one of the most important causes of bacterial infections transmitted through water and foods. Several factors are involved in the pathogenesis of E. coli. The aim of this study was to investigate the prevalence of E.coli strains carrying virulence gene ipaH isolated from different water sources in Alborz province.

Methods: This study was carried out in 2013. The research included all E.coli strains isolated from different surface water sources in Alborz province. E.coli isolates were detected and identified by standard microbiological and biochemical tests. The strains were evaluated for presence of virulence gene ipaH by PCR using specific primers. The PCR amplicons were visualized after electrophoresis and staining with ethidium bromide.

Results: Ninety nine E. coli strains were isolated and included in the study. The PCR results showed %88 of the strains harbored ipaH gene. There was any difference between detection rates of ipaH gene among the strains isolated from different water source. …

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