Academic journal article Iranian Journal of Public Health

Molecular Characterization of 4 Lytic Bacteriophages Specific for Salmonella Enteritidis

Academic journal article Iranian Journal of Public Health

Molecular Characterization of 4 Lytic Bacteriophages Specific for Salmonella Enteritidis

Article excerpt

Background: Progressing usage of various bacteriophages in genetic engineering, biotechnology and phage-therapy also using them as appropriate tools in order to determine type and rank of Barcia's focused attentions of most researches to introduce new types and vast application for them also. In researches, its required to introduce their new types and vari- ous usages and each invention opens a new vision in science expansion.

Methods: In order to separate bacteriophage, filtered sus- pension of bird samples added to fresh standard salmonellaen- teritidis culture and bacteriophage pure with frequent passag- es. It was studied host domain and genetic ingredients of At least 4separated lytic bacteriophage. After genome extraction, genomic type and pattern was determined in agar gel through electrophoresis. Separated genome of each bacteriophage was studied through polymerase chain reaction PCR about STX gen existence (in order to study toxin production gen transfer capability) and INT gen (to study lytic bacteriophage poten- tial probability conversion to lysogenic form. …

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