Academic journal article Iranian Journal of Public Health

Detection of Listeria Monocytogenes in Viable but Nonculturable State by 16s Rrna Gene Expression

Academic journal article Iranian Journal of Public Health

Detection of Listeria Monocytogenes in Viable but Nonculturable State by 16s Rrna Gene Expression

Article excerpt

Background: This study aimed to investigate RT-PCR for 16S rRNA as a reliable method for precise and specific detec- tion of VBNC state of Listeria monocytogenes.

Methods: For this purpose VBNC state of these bacteria was created in three environmental shocks: high osmotic shock, nutritional depauperation, cold temperature. The ac- tive and dead treatments were as positive and negative con- trol groups. The culturability af treatments were checked on BHI agar containing yeast extract and sodium pirovate. Via- bility of treatment confirm by syto9 fluorescent dye by fluo- rescent microscope. Then the RNA extraction, Dnase treat- ment, cDNA synthesis and PCR for 16S rRNA were done for all samples.

Results: The results showed that the 16S rRNA gene in via- ble bacteria as a positive control, VBNC state from high os- motic shock, VBNC state from cold temperature and VBNC state from nutritional depauperation express. …

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