Academic journal article Iranian Journal of Public Health

Pectinase Enzyme Derived from Aspergillus Fumigatus Is a Biocatalyst That Used Widely in Food Industry

Academic journal article Iranian Journal of Public Health

Pectinase Enzyme Derived from Aspergillus Fumigatus Is a Biocatalyst That Used Widely in Food Industry

Article excerpt

Background: Pectinase is used in fruit juice industry, cleans- ing oil industry, paper-making, textile, fermented tea and coffee, water and waste treatment.The main strategies that are used to enhance production of this enzyme is mutagene- sis or optimization By the experimental design. The purpose of this study is optimizing of pectinase enzyme production and rising of thermal stability is that.

Methods: In this study, optimization of enzyme production was performanced for the three fungi, Aspergillus fumigatus, Penicillium chryzogenum and Rhizopus oryzea by application of the factorial design which involves five factors, each at three levels. The five factors were carbon sources (whey, sugar, stevia) and ammonium sulfate, manganese sulfate, tempera- ture, and pH). Pectinase concentration was measured by the Miller method.

Results: The results showed that the optimum of enzyme production was at 32 °C, PH = 6, 3g / L manganese sulfate, 2. …

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