Academic journal article Iranian Journal of Public Health

Optimization of the Iron Bioleaching Parameters in Leptospirillum Ferriphilum UTMC 2299, Isolated from Gol-E-Gohar Iron Ore Mine

Academic journal article Iranian Journal of Public Health

Optimization of the Iron Bioleaching Parameters in Leptospirillum Ferriphilum UTMC 2299, Isolated from Gol-E-Gohar Iron Ore Mine

Article excerpt

Background: In this study, we evaluated some physical and chemical parameters to determine the optimum conditions for Biooxidizing of ferrus iron to the ferric form by Lepto- spirillum ferriphilum UTMC 2299.

Methods: Initially, the bacteria was cultured in 9K medium, pH 1.8 and incubated at 28 °C with shaking at 150 rpm in aerobic condition. In order to achieve the highest iron oxida- tion activity, the parameters including incubation time (3, 6. 9 and 15 day), particle size (45, 75, 106, 126 and 150 µm) initial pH (1.5, 1.75, 2, 2.5 and 3), initial inoculation volume (5, 7.5, 10 and 15% V/V), incubation temperature (28, 32, 37, 40 and 42 °C) and initial iron concentration (5, 7.5, 10, 15 and 20 g/l) were tested. Each test was performed in three repeats. Measurement of atomic absorption and total cell protein followed by Bradford test were used to evaluate the biooxida- tion and bacterial growth, respectively.

Results: The Gram-negative iron oxidizing bacteria, L. fer- riphilum was isolated from Gol-Gohar iron mine in Kerman and preserved in University of Tehran Microorganisms Col- lection (UTMC), under the accession number, UTMC 2299 in the previous study. Based o n atomic absorption and Brad- ford results, the optimum growth and iron oxidizing activity was observed in particle size of 45 µm, initial pH of 1. …

Search by... Author
Show... All Results Primary Sources Peer-reviewed

Oops!

An unknown error has occurred. Please click the button below to reload the page. If the problem persists, please try again in a little while.