Academic journal article Iranian Journal of Public Health

Expression of Synthetic Peptide of M2e-Ha2 of Influenza Virus on the Surface of Bacteriophage of M13 in Order to Increase Its Antigenicity

Academic journal article Iranian Journal of Public Health

Expression of Synthetic Peptide of M2e-Ha2 of Influenza Virus on the Surface of Bacteriophage of M13 in Order to Increase Its Antigenicity

Article excerpt

Background: The small peptides can expressed in multiple copies on the filamentous phage M13 or fd as amino- terminal fusions. These peptides are usually expressed with the phage coat protein pIII or pVIII. Such fusion peptides are able to induce humoral as well as cell-mediated immune responses, which makes phage particles an attractive antigen delivery system. The aim of current study was to display a synthetic peptide containing two epitopes of Influenza virus (from M2e and HA2 regions) on the surface of pVIII bacte- riophage of M13.

Methods: To express the peptide on the surface of bacterio- phage M13, the Influenza peptide gene and gVIII gene were amplified and joined to each other by Assembly PCR tech- nique. The constructed gene was then cloned into PIT2 phagemid vector and expressed in E. …

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