Academic journal article Iranian Journal of Public Health

Analytical Specificity and Sensitivity of Loop Mediated Isothermal Amplification Assay Targeting Com1 Gene of Coxiella Burnetii

Academic journal article Iranian Journal of Public Health

Analytical Specificity and Sensitivity of Loop Mediated Isothermal Amplification Assay Targeting Com1 Gene of Coxiella Burnetii

Article excerpt

Background: C.burnetii, the bacterium causing Q fever, is an obligate intracellular biosafety level 3 agent. Detection and quantification of these bacteria with conventional methods is time consuming and dangerous. Rapid detection of the agent improves the management of infection in natural or inten- tional epidemics. In the study, analytical properties of a com 1 gene based loop mediated isothermal amplification for de- tection of Q fever agent was evaluated.

Methods: LAMP primers were designed in accord with the com1 gene. Specificity of the assay was tested on genomic DNA of negative control bacteria. Sensitivity of the assay was calculated in serially diluted positive control plasmid con- taining PCR product of com1 gene from 100 billion to 10 particles. LAMP product was electrophoresis in 2% agaros gel (0.5X TBE) stained with Ethidium Bromide.

Results: The Electrophoresis of the LAMP products showed different size fragments in smear shape. No amplification signal observed in loop mediated isothermal amplification of negative control bacteria accompanied by specific C. …

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