Academic journal article Iranian Journal of Public Health

Protective Effect of Carotenoid Extraction from « Kocuria Asb107 » Bacteria on DNA Plasmid

Academic journal article Iranian Journal of Public Health

Protective Effect of Carotenoid Extraction from « Kocuria Asb107 » Bacteria on DNA Plasmid

Article excerpt

Background: In this study, the role of carotenoids extracted from this bacterium in free radicals scavenging and protec- tion of plasmid DNA was assayed.

Methods: In this study, oxidative damage on DNA induced by treatment of pUC18 with, hydrogen peroxide (H2O2) and tin chloride (SnCl2).The induced damage in plasmid DNA was carried out in the absence of extracted carotenoid from Kocuria ASB107, and also in the presence of various concen- trations of extraction in Dimethyl sulfoxide (DMSO) solvent. A double-strand plasmid DNA break was determined on the rate of conversion supercoiling double-strand DNA to nicked (open) circular and linear forms and create smear on gel agarose.

Results: Agarose gel analysis of treated plasmid DNA with SnCl2 showed that in the absence of carotenoid extract, one smear was formed, but with increasing concentration of ca- rotenoid extract from Kocuria, the rate of smear dropped and the bands related to Open Circular (OP) and Supercoil (SC) forms were determined. …

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