Academic journal article Iranian Journal of Public Health

In Vivo Bioactivity of Truncated Streptokinase Lacking 42 Amino Acids in C-Terminal Region

Academic journal article Iranian Journal of Public Health

In Vivo Bioactivity of Truncated Streptokinase Lacking 42 Amino Acids in C-Terminal Region

Article excerpt

Background: In the present study, the bioactivity of the recombinant streptokinase (rSK) was evaluated in vivo.

Methods: Streptokinase mutant gene, lacking 42 amino ac- ids, was cloned in pET32a. Expression of the rSK was con- firmed by western blotting and then purified by affinity chromatography. For in vivo investigation, blood clot was created in the jugular vein of rabbit after a general anesthesia. Clot formation was confirmed by Doppler ultrasound The rSK (4000 IU) was administered in marginal vein of rabbits' ear. The animals were kept under standard conditions. After 1 h, level of the D-dimer, in plasma of the animals was meas- ured and compared with those of the animals treated with a commercial whole length streptokinase (Heberkinase). Dis- tribution of data was assessed by Kolmogorov-Smirnov test and analyzed by independent-sample t-test. A P value of < 0.05 was defined as the level of significance.

Results: Doppler ultrasound showed clot formation in jugu- lar vein of the rabbits. In addition, there was a significant difference between d-dimer levels in plasma of the animals before and after the SK administrations, indicating the in vivo bioactivity of both truncated and commercial SKs (p < 0. …

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