Academic journal article Iranian Journal of Public Health

Microbial Synthesis of Silver Nanoparticle by Culture Supernatants of Alcaligenes Species Strain Ck-Cr6

Academic journal article Iranian Journal of Public Health

Microbial Synthesis of Silver Nanoparticle by Culture Supernatants of Alcaligenes Species Strain Ck-Cr6

Article excerpt

Background: Nanotechnology has dynamically developed as an important field of modern research with potential effects in electronic, chemistry and medicine.

Methods: After culturing the bacteria in the culture medium, supernatant phase was separated by centrifugation. Produc- tion of silver nanoparticles using bacterial supernatant phase was carried out by adding an aqueous concentrations of Ag- No3(1mM to 5mM) solution. The prepared mixtures were incubated at different temperatures (25 °C-50 °C) after 24 hour was absorbed. UV-Vis spectra of the mixtures of nano- particles were recorded in the range of 300-700 nm. Other characteristics of produced nanoparticles such as average particle size.XRD,DLS,zeta potential and FTIR were also determined.

Results: 16SRNA molecular techniques were used to charac- terization the strain type,also phylogenetic analysis showed that the most similar to Alcaligenes species.Biosynthesis of silver nanoparticles occurred at 50 and 70°C in various range concentrations of Agno3. …

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